Pancreatic regenerating gene I and acinar cell differentiation: influence on cellular lineage.

ABSTRACT:

Objectives

Pancreatic regenerating gene I (reg I) has been implicated in cellular differentiation. Acinar cells can transdifferentiate into other pancreatic-derived cells, and we postulated that changes in intracellular levels of reg I would affect the state of differentiation.

Methods

We transfected AR42J cells with a plasmid containing the entire coding sequence of reg I and isolated clones with complementary DNA in sense (SS) or antisense (AS) orientation. Levels of messenger RNA (mRNA) and protein expression were examined by Western blotting and real-time polymerase chain reaction.

Results

Expression of reg I was confirmed in SS or AS clones. AR42J transfected with SS demonstrated more acinarlike phenotype, whereas those transfected with AS showed a less different

SUBMITTER: Sanchez D 

PROVIDER: S-EPMC2702698 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature