Systemic analysis of the response of Aspergillus niger to ambient pH.

ABSTRACT:

Background

The filamentous fungus Aspergillus niger is an exceptionally efficient producer of organic acids, which is one of the reasons for its relevance to industrial processes and commercial importance. While it is known that the mechanisms regulating this production are tied to the levels of ambient pH, the reasons and mechanisms for this are poorly understood.

Methods

To cast light on the connection between extracellular pH and acid production, we integrate results from two genome-based strategies: A novel method of genome-scale modeling of the response, and transcriptome analysis across three levels of pH.

Results

With genome scale modeling with an optimization for extracellular proton-production, it was possible to reproduce the preferred pH levels for citrate

SUBMITTER: Andersen MR 

PROVIDER: S-EPMC2718513 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature