Genome-wide screen of promoter methylation identifies novel markers in melanoma.

ABSTRACT: DNA methylation is an important component of epigenetic modifications, which influences the transcriptional machinery aberrant in many human diseases. In this study we present the first genome-wide integrative analysis of promoter methylation and gene expression for the identification of methylation markers in melanoma. Genome-wide promoter methylation and gene expression of eight early-passage human melanoma cell strains were compared with newborn and adult melanocytes. We used linear mixed effect models (LME) in combination with a series of filters based on the localization of promoter methylation relative to the transcription start site, overall promoter CpG content, and differential gene expression to discover DNA methylation markers. This approach identified 76 markers, of which 68 we

SUBMITTER: Koga Y 

PROVIDER: S-EPMC2720187 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature