Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens.

ABSTRACT:

Background

The role of the RNA polymerase sigma factor RpoN in regulation of gene expression in Geobacter sulfurreducens was investigated to better understand transcriptional regulatory networks as part of an effort to develop regulatory modules for genome-scale in silico models, which can predict the physiological responses of Geobacter species during groundwater bioremediation or electricity production.

Results

An rpoN deletion mutant could not be obtained under all conditions tested. In order to investigate the regulon of the G. sulfurreducens RpoN, an RpoN over-expression strain was made in which an extra copy of the rpoN gene was under the control of a taclac promoter. Combining both the microarray transcriptome analysis and the computational prediction revealed that th

SUBMITTER: Leang C 

PROVIDER: S-EPMC2725144 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature