Directed sample interrogation utilizing an accurate mass exclusion-based data-dependent acquisition strategy (AMEx).

ABSTRACT: The ability to perform thorough sampling is of critical importance when using mass spectrometry to characterize complex proteomic mixtures. A common approach is to reinterrogate a sample multiple times by LC-MS/MS. However, the conventional data-dependent acquisition methods that are typically used in proteomics studies will often redundantly sample high-intensity precursor ions while failing to sample low-intensity precursors entirely. We describe a method wherein the masses of successfully identified peptides are used to generate an accurate mass exclusion list such that those precursors are not selected for sequencing during subsequent analyses. We performed multiple concatenated analytical runs to sample a complex cell lysate, using either accurate mass exclusion-based data-dependent a

SUBMITTER: Rudomin EL 

PROVIDER: S-EPMC2753172 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature