Activation of CCR9/CCL25 in cutaneous melanoma mediates preferential metastasis to the small intestine.

ABSTRACT:

Purpose

Specific chemokines and their respective receptors have been implicated in distant tumor cell metastasis. Cutaneous melanoma has a distinct pattern of metastasis, preferentially targeting the submucosa of the small intestine. However, the underlying pathogenic mechanism remains unknown. Migration of CCR9(+) lymphocytes to the small intestine is known to occur in response to the chemoattractant effects of CCL25 (thymus-expressed chemokine). The integrin heterodimers alphabeta are also known to be important mediators of cellular adhesion. We hypothesize that the mechanism of small intestinal metastasis by melanoma is via the CCR9-CCL25 axis and specific integrins.

Experimental design

Quantitative reverse transcription-PCR, flow cytometry, and immunohistochemistry were

SUBMITTER: Amersi FF 

PROVIDER: S-EPMC2760931 | biostudies-literature | 2008 Feb

REPOSITORIES: biostudies-literature