Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates.

ABSTRACT:

Aim

To confirm the presence of recombination, full-length hepatitis B virus (HBV) from chronic patients was sequenced and analyzed.

Methods

Full-length HBV genomes from 12 patients were amplified and sequenced in an automated sequencer. Phylogenetic analysis was carried out on full-length, Core and preS2/Surface regions using MEGA software. SimPlot Boot Scanning and amino acid sequence analysis were performed for confirmation of recombination.

Results

Eight patients were infected with genotype D strain; one patient with genotype A and three patients had genotype A and D recombination; two of them had cirrhosis and one had hepatocellular carcinoma. Phylogenetic analysis of core and preS2/surface regions separately showed evidence of genotype A and D recombination. The

SUBMITTER: Chauhan R 

PROVIDER: S-EPMC2761587 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature