HIV drug resistance surveillance using pooled pyrosequencing.

ABSTRACT:

Background

Surveillance for HIV transmitted drug resistance (TDR) is performed using HIV genotype results from individual specimens. Pyrosequencing, through its massive parallel sequencing ability, can analyze large numbers of specimens simultaneously. Instead of using pyrosequencing conventionally, to sequence a population of viruses within an individual, we interrogated a single combined pool of surveillance specimens to demonstrate that it is possible to determine TDR rates in HIV protease from a population of individuals.

Methodology/principal findings

The protease region from 96 treatment naïve, HIV+ serum specimens was genotyped using standard Sanger sequencing method. The 462 bp protease amplicons from these specimens were pooled in equimolar concentrations and re-seq

SUBMITTER: Ji H 

PROVIDER: S-EPMC2822863 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature