Time-series gene expression profiles in AGS cells stimulated with Helicobacter pylori.

ABSTRACT:

Aim

To extend the knowledge of the dynamic interaction between Helicobacter pylori (H. pylori) and host mucosa.

Methods

A time-series cDNA microarray was performed in order to detect the temporal gene expression profiles of human gastric epithelial adenocarcinoma cells infected with H. pylori. Six time points were selected to observe the changes in the model. A differential expression profile at each time point was obtained by comparing the microarray signal value with that of 0 h. Real-time polymerase chain reaction was subsequently performed to evaluate the data quality.

Results

We found a diversity of gene expression patterns at different time points and identified a group of genes whose expression levels were significantly correlated with several important immune

SUBMITTER: You YH 

PROVIDER: S-EPMC2842531 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature