Natural taurine promotes apoptosis of human hepatic stellate cells in proteomics analysis.

ABSTRACT:

Aim

To study the differential expression of proteins between natural taurine treated hepatic stellate cells and controls, and investigate the underlying regulatory mechanism of natural taurine in inhibiting hepatic fibrosis.

Methods

A proteomic strategy combining two-dimensional gel electrophoresis and ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) was used to study the differential expression of proteins and Western blotting was used to validate the results. Gene ontology (GO) method was utilized to analyze the functional enrichment of differentially expressed proteins. Flow cytometry was performed to compare the apoptosis rate between taurine-treated and untreated hepatic stellate cells (HSCs).

Results

Ninet

SUBMITTER: Deng X 

PROVIDER: S-EPMC2856835 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature