Low aerobic mitochondrial energy metabolism in poorly- or undifferentiated neuroblastoma.

ABSTRACT:

Background

Succinate dehydrogenase (SDH) has been associated with carcinogenesis in pheochromocytoma and paraganglioma. In the present study we investigated components of the oxidative phosphorylation system in human neuroblastoma tissue samples.

Methods

Spectrophotometric measurements, immunohistochemical analysis and Western blot analysis were used to characterize the aerobic mitochondrial energy metabolism in neuroblastomas (NB).

Results

Compared to mitochondrial citrate synthase, SDH activity was severely reduced in NB (n = 14) versus kidney tissue. However no pathogenic mutations could be identified in any of the four subunits of SDH. Furthermore, no genetic alterations could be identified in the two novel SDH assembly factors SDHAF1 and SDH5. Alterations in gene

SUBMITTER: Feichtinger RG 

PROVIDER: S-EPMC2861660 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature