Direct detection of nitroxyl in aqueous solution using a tripodal copper(II) BODIPY complex.

ABSTRACT: The synthesis, photophysical properties, and a biological application of BODIPY-triazole 1 (BOT1) are described. BOT1 juxtaposes a BODIPY fluorophore with a tripodal ligand platform via a triazole bridge. The triazole linker is afforded by azide-alkyne "click" chemistry and comprises the third arm of the tripodal architecture. BOT1 binds Cu(2+) in aqueous solution to form a Cu(II)[BOT1] complex in which emission from the BODIPY moiety is efficiently quenched. This complex exhibits a prompt turn-on response when exposed to nitroxyl, and this emission response is specific to HNO over other reactive nitrogen and oxygen species. Notably, Cu(II)[BOT1] does not fluoresce in the presence of nitric oxide, making this system the first discrete molecular probe capable of detecting HNO over NO under

SUBMITTER: Rosenthal J 

PROVIDER: S-EPMC2866442 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature