Dataset Information

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

ABSTRACT: A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.

SUBMITTER: Nauwelaerts K

PROVIDER: S-EPMC290278 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

Nauwelaerts Koen K Vastmans Karen K Froeyen Matheus M Kempeneers Veerle V Rozenski Jef J Rosemeyer Helmut H Van Aerschot Arthur A Busson Roger R Lacey Jeffrey C JC Efimtseva Ekaterina E Mikhailov Sergey S Lescrinier Eveline E Herdewijn Piet P

Nucleic acids research 20031201 23

A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means ...[more]

PMID: 14627809

Similar Datasets

Project description:ObjectivesMany trials investigate potential effects of treatments for coronavirus disease 2019. To provide sufficient information for all involveddecision-makers (clinicians, public health authorities, and drug regulatory agencies), a multiplicity of endpoints must be considered. The objectives are to provide hands-on statistical guidelines for harmonizing heterogeneous endpoints in coronavirus disease 2019 clinical trials.DesignRandomized controlled trials for patients infected with coronavirus disease 2019.SettingGeneral methods that apply to any randomized controlled trial for patients infected with coronavirus disease 2019.PatientsCoronavirus disease 2019 positive individuals.InterventionsNone.Measurements and main resultsWe develop a multistate model that is based on hospitalization, mechanical ventilation, death, and discharge. These events are both categories of the ordinal endpoint recommended by the World Health Organization and also within the core outcome set of the Core Outcome Measures in Effectiveness Trials initiative for coronavirus disease 2019 trials. To support our choice of states in the multistate model, we also perform a brief review of registered coronavirus disease 2019 clinical trials. Based on the multistate model, we give recommendation for compact, informative illustration of time-dynamic treatment effects and explorative statistical analysis. A majority of coronavirus disease 2019 clinical trials collect information on mechanical ventilation, hospitalization, and death. Using reconstructed and real data of coronavirus disease 2019 trials, we show how a stacked probability plot provides a detailed understanding of treatment effects on the patients' course of hospital stay. It contributes to harmonizing multiple endpoints and differing lengths of follow-up both within and between trials.ConclusionsAll ongoing clinical trials should include a stacked probability plot in their statistical analysis plan as descriptive analysis. While primary analysis should be on an early endpoint with appropriate capability to be a surrogate (parameter), our multistate model provides additional detailed descriptive information and links results within and between coronavirus disease 2019 trials.

Dataset Information

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

Publications

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets