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Acrylamide-dT: a polymerisable nucleoside for DNA incorporation.

ABSTRACT: The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acrylamide polymers was evidenced by gel electrophoresis. The Acrylamide-dT functional handle promises to be an ideal synthon for preparing DNA-polymer hybrids for use in various macromolecular materials applications.

SUBMITTER: Allabush F

PROVIDER: S-EPMC9072585 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

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Acrylamide-dT: a polymerisable nucleoside for DNA incorporation.

Allabush Francia F Mendes Paula M PM Tucker James H R JHR

RSC advances 20191003 54

The synthesis of a novel modified nucleoside phosphoramidite, Acrylamide-dT-CE phosphoramidite, obtained in three steps from commercially available starting materials, is reported. It was readily incorporated into thrombin binding aptamer (TBA) sequences using automated solid-phase synthesis under ultra-mild conditions, with the modification shown not to adversely affect duplex stability, G-quadruplex structure, or thrombin binding. The reaction and integration of the modified strands with acryl ...[more]

PMID: 35527933

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