Identification of proteins related to the stress response in Enterococcus faecalis V583 caused by bovine bile.

ABSTRACT:

Background

Enterococcus faecalis is an opportunistic pathogen and one of the most important causes of hospital infections. Bile acids are a major stress factor bacteria have to cope with in order to colonize and survive in the gastro-intestinal tract. The aim of this study was to investigate the effects of bile acids on the intracellular proteome of E. faecalis V583.

Results

The proteomes of cells challenged with 1% bile were analyzed after 20 - 120 minutes exposure, using 2D gel electrophoresis and mass spectrometry. Among the approximately 500 observed proteins, 53 unique proteins were found to be regulated in response to bile and were identified with mass spectrometry. The identified proteins belonged to nine different functional classes, including fatty acid- and phospho

SUBMITTER: Bohle LA 

PROVIDER: S-EPMC2907315 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature