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Synthesis and g-quadruplex-binding properties of defined acridine oligomers.

ABSTRACT: The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectral changes were used to calculate the binding constants (K). Log K were found to be in the order of 4-6.

SUBMITTER: Ferreira R

PROVIDER: S-EPMC2915818 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Synthesis and g-quadruplex-binding properties of defined acridine oligomers.

Ferreira Rubén R Aviñó Anna A Pérez-Tomás Ricardo R Gargallo Raimundo R Eritja Ramon R

Journal of nucleic acids 20100613

The synthesis of oligomers containing two or three acridine units linked through 2-aminoethylglycine using solid-phase methodology is described. Subsequent studies on cell viability showed that these compounds are not cytotoxic. Binding to several DNA structures was studied by competitive dialysis, which showed a clear affinity for DNA sequences that form G-quadruplexes and parallel triplexes. The fluorescence spectra of acridine oligomers were affected strongly upon binding to DNA. These spectr ...[more]

PMID: 20725626

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