Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia.

ABSTRACT:

Background

Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and without consideration for the subcellular distribution of the perturbed actin cytoskeleton.

Results

Here, we in addition to toxins use conditional expression of the major actin regulatory protein LIM kinase-1 (LIMK1), and shRNA knock-down of cofilin to modulate the cellular F/G-actin ratio in the Ra2 microglia cell line, and we use Fluorescence Recovery after Photobleaching (FRAP) in β-actin-YFP-transduced cells to obtain a dynamic measure of actin recovery ra

SUBMITTER: Rasmussen I 

PROVIDER: S-EPMC2944333 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature