Novel glycoside hydrolases identified by screening a Chinese Holstein dairy cow rumen-derived metagenome library.

ABSTRACT: One clone encoding glycoside hydrolases was identified through functional screening of a rumen bacterial artificial chromosome (BAC) library. Of the 68 open reading frames (ORFs) predicted, one ORF encodes a novel endo-?-1,4-xylanase with two catalytic domains of family GH43 and two cellulose-binding modules (CBMs) of family IV. Partial characterization showed that this endo-xylanase has a greater specific activity than a number of other xylanases over a wide temperature range at neutral pH and could be useful in some industrial applications.

SUBMITTER: Zhao S 

PROVIDER: S-EPMC2950470 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature