Antibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle.

ABSTRACT:

Background

Bovine tuberculosis, caused by Mycobacterium bovis, afflicts approximately 50 million cattle worldwide and is detected by the tuberculin skin test (TST). While it has long been recognized that purified protein derivative (PPD) tuberculin is composed of a mixture of M. bovis derived protein components, little is known about the quality, relative quantity and identity of the proteins that make up PPD tuberculin. We manufactured a sterile filtered PPD tuberculin (SF-PPD) from a nine-week-old M. bovis culture supernatant in order to characterise the culture filtrate proteins (CFP) which make up M. bovis PPD tuberculin and to compare the antibody response of M. bovis infected versus M. bovis sensitized cattle.

Results

SF-PPD resolved into approximately 200 discrete spo

SUBMITTER: Rennie B 

PROVIDER: S-EPMC2994848 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature