Tiling histone H3 lysine 4 and 27 methylation in zebrafish using high-density microarrays.

ABSTRACT:

Background

Uncovering epigenetic states by chromatin immunoprecipitation and microarray hybridization (ChIP-chip) has significantly contributed to the understanding of gene regulation at the genome-scale level. Many studies have been carried out in mice and humans; however limited high-resolution information exists to date for non-mammalian vertebrate species.

Principal findings

We report a 2.1-million feature high-resolution Nimblegen tiling microarray for ChIP-chip interrogations of epigenetic states in zebrafish (Danio rerio). The array covers 251 megabases of the genome at 92 base-pair resolution. It includes ∼15 kb of upstream regulatory sequences encompassing all RefSeq promoters, and over 5 kb in the 5' end of coding regions. We identify with high reproducibility, in

SUBMITTER: Lindeman LC 

PROVIDER: S-EPMC3004928 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature