Enhanced energy expenditure, glucose utilization, and insulin sensitivity in VAMP8 null mice.

ABSTRACT:

Objective

Previous studies have demonstrated that the VAMP8 protein plays a complex role in the control of granule secretion, transport vesicle trafficking, phagocytosis, and endocytosis. The present study was aimed to investigate the role of VAMP8 in mediating GLUT4 trafficking and therefore insulin action in mice.

Research design and methods

Physiological parameters were measured using Oxymax indirect calorimetry system in 12-week-old VAMP8 null mice. Dynamic analysis of glucose homeostasis was assessed using euglycemic-hyperinsulinemic clamp coupled with tracer radioactively labeled 2-deoxyglucose. Insulin stimulated GLUT4 protein expressions on muscle cell surface were examined by immunofluorescence microscopy.

Results

VAMP8 null mice display reduced adiposity wit

SUBMITTER: Zong H 

PROVIDER: S-EPMC3012186 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature