Unknown

Dataset Information

0

Histone deacetylase regulates high mobility group A2-targeting microRNAs in human cord blood-derived multipotent stem cell aging.


ABSTRACT: Cellular senescence involves a reduction in adult stem cell self-renewal, and epigenetic regulation of gene expression is one of the main underlying mechanisms. Here, we observed that the cellular senescence of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) caused by inhibition of histone deacetylase (HDAC) activity leads to down-regulation of high mobility group A2 (HMGA2) and, on the contrary, to up-regulation of p16(INK)?(A), p21(CIP)¹(/WAF)¹ and p27(KIP)¹. We found that let-7a1, let-7d, let-7f1, miR-23a, miR-26a and miR-30a were increased during replicative and HDAC inhibitor-mediated senescence of hUCB-MSCs by microRNA microarray and real-time quantitative PCR. Furthermore, the configurations of chromatins beading on these miRNAs were prone to transcriptional activation during HDAC inhibitor-mediated senescence. We confirmed that miR-23a, miR-26a and miR-30a inhibit HMGA2 to accelerate the progress of senescence. These findings suggest that HDACs may play important roles in cellular senescence by regulating the expression of miRNAs that target HMGA2 through histone modification.

SUBMITTER: Lee S 

PROVIDER: S-EPMC3016490 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

Histone deacetylase regulates high mobility group A2-targeting microRNAs in human cord blood-derived multipotent stem cell aging.

Lee Seunghee S   Jung Ji-Won JW   Park Sang-Bum SB   Roh Kyounghwan K   Lee Su Yeon SY   Kim Ju Han JH   Kang Soo-Kyung SK   Kang Kyung-Sun KS  

Cellular and molecular life sciences : CMLS 20100721 2


Cellular senescence involves a reduction in adult stem cell self-renewal, and epigenetic regulation of gene expression is one of the main underlying mechanisms. Here, we observed that the cellular senescence of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs) caused by inhibition of histone deacetylase (HDAC) activity leads to down-regulation of high mobility group A2 (HMGA2) and, on the contrary, to up-regulation of p16(INK)⁴(A), p21(CIP)¹(/WAF)¹ and p27(KIP)¹. We found tha  ...[more]

Similar Datasets

| S-EPMC4382927 | biostudies-literature
| S-EPMC527137 | biostudies-literature
| S-EPMC3481173 | biostudies-literature
| S-EPMC4914320 | biostudies-literature
| S-EPMC3133373 | biostudies-literature
| S-EPMC4197860 | biostudies-literature
| S-EPMC6260922 | biostudies-literature
| S-EPMC9966875 | biostudies-literature
| S-EPMC6827107 | biostudies-literature
| S-EPMC6160007 | biostudies-literature