Post-transcriptional regulation in the myo1Δ mutant of Saccharomyces cerevisiae.

ABSTRACT:

Background

Saccharomyces cerevisiae myosin type II-deficient (myo1Δ) strains remain viable and divide, despite the absence of a cytokinetic ring, by activation of the PKC1-dependent cell wall integrity pathway (CWIP). Since the myo1Δ transcriptional fingerprint is a subset of the CWIP fingerprint, the myo1Δ strain may provide a simplified paradigm for cell wall stress survival.

Results

To explore the post-transcriptional regulation of the myo1Δ stress response, 1,301 differentially regulated ribosome-bound mRNAs were identified by microarray analysis of which 204 were co-regulated by transcription and translation. Four categories of mRNA were significantly affected - protein biosynthesis, metabolism, carbohydrate metabolism, and unknown functions. Nine genes of the 20 CWIP f

SUBMITTER: Rivera-Ruiz ME 

PROVIDER: S-EPMC3017085 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature