Mechanisms of actions of hydrogen sulphide on rat distal colonic epithelium.

ABSTRACT:

Background and purpose

The aim of this study was to clarify the mechanisms by which hydrogen sulphide (H₂S) affects ion secretion across rat distal colonic epithelium.

Experimental approach

Changes in short-circuit current induced by the H₂S-donor, sodium hydrosulphide (NaHS; 10 mmol·L⁻¹), were measured in Ussing chambers after permeabilization of the apical membrane with nystatin. Cytosolic Ca²(+) concentration ([Ca²(+)](i)) and Ca²(+) in intracellular stores were measured with fluorescent dyes. Changes in mitochondrial membrane potential were estimated with rhodamine 123.

Key results

NaHS had a biphasic effect on overall currents across the basolateral membrane: an initial inhibition followed by a secondary stimulation. Both a scilliroside-sensitive action on the Na

SUBMITTER: Pouokam E 

PROVIDER: S-EPMC3031060 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature