Label-free peptide profiling of Orbitrap™ full mass spectra.

ABSTRACT:

Background

We developed a new version of the open source software package Peptrix that can yet compare large numbers of Orbitrap™ LC-MS data. The peptide profiling results for Peptrix on MS1 spectra were compared with those obtained from a small selection of open source and commercial software packages: msInspect, Sieve™ and Progenesis™. The properties compared in these packages were speed, total number of detected masses, redundancy of masses, reproducibility in numbers and CV of intensity, overlap of masses, and differences in peptide peak intensities. Reproducibility measurements were taken for the different MS1 software applications by measuring in triplicate a complex peptide mixture of immunoglobulin on the Orbitrap™ mass spectrometer. Values of peptide masses detected from t

SUBMITTER: Titulaer MK 

PROVIDER: S-EPMC3042405 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature