Regulatory haplotypes in ARG1 are associated with altered bronchodilator response.

ABSTRACT:

Rationale

β₂-agonists, the most common treatment for asthma, have a wide interindividual variability in response, which is partially attributed to genetic factors. We previously identified single nucleotide polymorphisms in the arginase 1 (ARG1) gene, which are associated with β₂-agonist bronchodilator response (BDR).

Objectives

To identify cis-acting haplotypes in the ARG1 locus that are associated with BDR in patients with asthma and regulate gene expression in vitro.

Methods

We resequenced ARG1 in 96 individuals and identified three common, 5' haplotypes (denoted 1, 2, and 3). A haplotype-based association analysis of BDR was performed in three independent, adult asthma drug trial populations. Next, each haplotype was cloned into vectors containing a luciferase rep

SUBMITTER: Duan QL 

PROVIDER: S-EPMC3056223 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature