Biomarkers of human oocyte developmental competence expressed in cumulus cells before ICSI: a preliminary study.

ABSTRACT:

Purpose

To identify reliable genomic biomarkers expressed in cumulus cells that accurately and non-invasively predict the oocyte developmental competence and reinforce the already used morphological criteria.

Methods

Eight consenting patients were selected for ovarian stimulation and ICSI procedures. Cumulus-oocyte complexes were transvaginally punctured and individually selected based on both good morphological criteria and high zona pellucida birefringence. Following ICSI, two 3-day embryos per patient were transferred. Pregnancy outcome was recorded and proven implantation was thereafter confirmed. Differential gene expression was assessed using two microarray platforms. Further real-time PCR validation, Ingenuity pathways analysis and intra-patient analysis were performe

SUBMITTER: Assidi M 

PROVIDER: S-EPMC3059525 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature