Assigning spectrum-specific P-values to protein identifications by mass spectrometry.

ABSTRACT:

Motivation

Although many methods and statistical approaches have been developed for protein identification by mass spectrometry, the problem of accurate assessment of statistical significance of protein identifications remains an open question. The main issues are as follows: (i) statistical significance of inferring peptide from experimental mass spectra must be platform independent and spectrum specific and (ii) individual spectrum matches at the peptide level must be combined into a single statistical measure at the protein level.

Results

We present a method and software to assign statistical significance to protein identifications from search engines for mass spectrometric data. The approach is based on asymptotic theory of order statistics. The parameters of the asympto

SUBMITTER: Spirin V 

PROVIDER: S-EPMC3072553 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature