Control of African swine fever virus replication by small interfering RNA targeting the A151R and VP72 genes.

ABSTRACT:

Background

African swine fever virus (ASFV) is the unique member of the Asfarviridae family and Asfivirus genus. It is an enveloped double-stranded DNA arbovirus that replicates in the cell cytoplasm, similar to poxviruses. There is no vaccine and no treatment available to control this virus.

Methods

We describe the use of small interfering RNA (siRNA) targeting the A151R and VP72 (B646L) genes to control the ASFV replication in vitro.

Results

Results suggest that siRNA targeting the A151R and VP72 genes can reduce both the virus replication and its levels of messenger RNA transcripts. The reduction was up to 4 log(10) copies on the virus titre and up to 3 log(10) copies on virus RNA transcripts levels. The combination of multiple siRNA did not improve the antiviral e

SUBMITTER: Keita D 

PROVIDER: S-EPMC3101891 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature