BTI1, an azoreductase with pH-dependent substrate specificity.

ABSTRACT: The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange.

SUBMITTER: Johansson HE 

PROVIDER: S-EPMC3131635 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature