Natural killer T cell-derived IL-17 mediates lung ischemia-reperfusion injury.

ABSTRACT:

Rationale

We recently implicated a role for CD4(+) T cells and demonstrated elevated IL-17A expression in lung ischemia-reperfusion (IR) injury. However, identification of the specific subset of CD4(+) T cells and their mechanistic role in IR injury remains unknown.

Objectives

We tested the hypothesis that invariant natural killer T (iNKT) cells mediate lung IR injury via IL-17A signaling.

Methods

Mice underwent lung IR via left hilar ligation. Pulmonary function was measured using an isolated lung system. Lung injury was assessed by measuring edema (wet/dry weight) and vascular permeability (Evans blue dye). Inflammation was assessed by measuring proinflammatory cytokines in lungs, and neutrophil infiltration was measured by immunohistochemistry and myeloperoxidase l

SUBMITTER: Sharma AK 

PROVIDER: S-EPMC3137143 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature