The microbial detection array combined with random Phi29-amplification used as a diagnostic tool for virus detection in clinical samples.

ABSTRACT: A common technique used for sensitive and specific diagnostic virus detection in clinical samples is PCR that can identify one or several viruses in one assay. However, a diagnostic microarray containing probes for all human pathogens could replace hundreds of individual PCR-reactions and remove the need for a clear clinical hypothesis regarding a suspected pathogen. We have established such a diagnostic platform for random amplification and subsequent microarray identification of viral pathogens in clinical samples. We show that Phi29 polymerase-amplification of a diverse set of clinical samples generates enough viral material for successful identification by the Microbial Detection Array, demonstrating the potential of the microarray technique for broad-spectrum pathogen detection. We co

SUBMITTER: Erlandsson L 

PROVIDER: S-EPMC3154197 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature