Quantitative characterization and analysis of the dynamic NF-κB response in microglia.

ABSTRACT:

Background

Activation of the NF-κB transcription factor and its associated gene expression in microglia is a key component in the response to brain injury. Its activation is dynamic and is part of a network of biochemical species with multiple feedback regulatory mechanisms. Mathematical modeling, which has been instrumental for understanding the NF-κB response in other cell types, offers a valuable tool to investigate the regulation of NF-κB activation in microglia at a systems level.

Results

We quantify the dynamic response of NF-κB activation and activation of the upstream kinase IKK using ELISA measurements of a microglial cell line following treatment with the pro-inflammatory cytokine TNFα. A new mathematical model is developed based on these data sets using a modular

SUBMITTER: Sheppard PW 

PROVIDER: S-EPMC3158563 | biostudies-literature | 2011 Jul

REPOSITORIES: biostudies-literature