Molecular profiling indicates orthotopic xenograft of glioma cell lines simulate a subclass of human glioblastoma.

ABSTRACT: Cell line models have been widely used to investigate glioblastoma multiforme (GBM) pathobiology and in the development of targeted therapies. However, GBM tumours are molecularly heterogeneous and how cell lines can best model that diversity is unknown. In this report, we investigated gene expression profiles of three preclinical growth models of glioma cell lines, in vitro and in vivo as subcutaneous and intracerebral xenografts to examine which cell line model most resembles the clinical samples. Whole genome DNA microarrays were used to profile gene expression in a collection of 25 high-grade glioblastomas, and comparisons were made to profiles of cell lines under three different growth models. Hierarchical clustering revealed three molecular subtypes of the glioblastoma patient sample

SUBMITTER: Shankavaram UT 

PROVIDER: S-EPMC3164941 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature