Clinically relevant orthotopic xenograft models of patient-derived glioblastoma in zebrafish.

ABSTRACT: An accurate prediction of the intracranial infiltration tendency and drug response of individual glioblastoma (GBM) cells is essential for personalized prognosis and treatment for this disease. However, the clinical utility of mouse patient-derived orthotopic xenograft (PDOX) models remains limited given current technical constraints, including difficulty in generating sufficient sample numbers from small tissue samples and a long latency period for results. To overcome these issues, we established zebrafish GBM xenografts of diverse origin, which can tolerate intracranial engraftment and maintain their unique histological features. Subsequent single-cell RNA-sequencing (scRNA-seq) analysis confirmed significant transcriptional identity to that of invading GBM microtumors observed in the p

SUBMITTER: Ai X 

PROVIDER: S-EPMC9066514 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature