Tunisian Toxoplasma gondii strains genotyping by the use of AK69 marker.

ABSTRACT:

Background

Clinical manifestation due to infection by Toxoplasma gondii is closely linked to the infecting strain of the parasite. Several genetic markers are available to determinate its genotype but few of them are able to discriminate between the three predominant lineages, namely types I, II and III. The number of markers decreases when atypical, recombinant/mixed genotypes need to be identified.

Findings

In our study, the contribution of sequence polymorphisms in the AK69 gene as typing markers for T. gondii was investigated for the first time in an epidemiological study. The coding region of the marker was amplified, sequenced and aligned for different Toxoplasma strains. The identified nucleotide polymorphism at 12 positions was able to highly discriminate between the

SUBMITTER: Boughattas S 

PROVIDER: S-EPMC3177785 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature