The role of codon selection in regulation of translation efficiency deduced from synthetic libraries.

ABSTRACT:

Background

Translation efficiency is affected by a diversity of parameters, including secondary structure of the transcript and its codon usage. Here we examine the effects of codon usage on translation efficiency by re-analysis of previously constructed synthetic expression libraries in Escherichia coli.

Results

We define the region in a gene that takes the longest time to translate as the bottleneck. We found that localization of the bottleneck at the beginning of a transcript promoted a high level of expression, especially if the computed dwell time of the ribosome within this region was sufficiently long. The location and translation time of the bottleneck were not correlated with the cost of expression, approximated by the fitness of the host cell, yet utilization of sp

SUBMITTER: Navon S 

PROVIDER: S-EPMC3188794 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature