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BMPs functionally replace Klf4 and support efficient reprogramming of mouse fibroblasts by Oct4 alone.


ABSTRACT: Generation of induced pluripotent stem cells by defined factors has become a useful model to investigate the mechanism of reprogramming and cell fate determination. However, the precise mechanism of factor-based reprogramming remains unclear. Here, we show that Klf4 mainly acts at the initial phase of reprogramming to initiate mesenchymal-to-epithelial transition and can be functionally replaced by bone morphogenetic proteins (BMPs). BMPs boosted the efficiency of Oct4/Sox2-mediated reprogramming of mouse embryonic fibroblasts (MEFs) to ?1%. BMPs also promoted single-factor Oct4-based reprogramming of MEFs and tail tibial fibroblasts. Our studies clarify the contribution of Klf4 in reprogramming and establish Oct4 as a singular setter of pluripotency in differentiated cells.

SUBMITTER: Chen J 

PROVIDER: S-EPMC3193408 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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BMPs functionally replace Klf4 and support efficient reprogramming of mouse fibroblasts by Oct4 alone.

Chen Jiekai J   Liu Jing J   Yang Jiaqi J   Chen You Y   Chen Jing J   Ni Su S   Song Hong H   Zeng Lingwen L   Ding Ke K   Ding Ke K   Pei Duanqing D  

Cell research 20101207 1


Generation of induced pluripotent stem cells by defined factors has become a useful model to investigate the mechanism of reprogramming and cell fate determination. However, the precise mechanism of factor-based reprogramming remains unclear. Here, we show that Klf4 mainly acts at the initial phase of reprogramming to initiate mesenchymal-to-epithelial transition and can be functionally replaced by bone morphogenetic proteins (BMPs). BMPs boosted the efficiency of Oct4/Sox2-mediated reprogrammin  ...[more]

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