Co-purification of Mac-2 binding protein with galectin-3 and association with prostasomes in human semen.

ABSTRACT:

Background

Prostasomes are exosome-like vesicles that are secreted by the prostate and incorporated into semen during ejaculation. Human prostasomes are proposed to function in regulation of sperm function, immunosuppression, and prostate cancer progression. Previously, we identified galectin-3 on the surface of prostasomes. Galectin-3 is a β-galactoside binding protein involved in immunomodulation, cell interactions, and cancer progression, including prostate cancer. Functional characterization of galectin-3 in a given biological environment includes identification of its target glycoprotein ligands.

Methods

Candidate galectin-3 ligands in prostasomes were identified by tandem mass spectrometry of proteins that co-purified with galectin-3 during lactose affinity chromatogra

SUBMITTER: Block AS 

PROVIDER: S-EPMC3210485 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature