Functional interaction between TRP4 and CFTR in mouse aorta endothelial cells.
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ABSTRACT: Background
This study describes the functional interaction between the putative Ca2+ channel TRP4 and the cystic fibrosis transmembrane conductance regulator, CFTR, in mouse aorta endothelium (MAEC).Results
MAEC cells express CFTR transcripts as shown by RT-PCR analysis. Application of a phosphorylating cocktail activated a Cl- current with characteristics similar to those of CFTR mediated currents in other cells types (slow activation by cAMP, absence of rectification, block by glibenclamide). The current is present in trp4 +/+ MAEC, but not in trp4 -/- cells, although the expression of CFTR seems unchanged in the trp4 deficient cells as judged from RT-PCR analysis.Conclusions
It is concluded that TRP4 is necessary for CFTR activation in endothelium, possibly by providing a scaffold for the formation of functional CFTR channels.
SUBMITTER: Wei L
PROVIDER: S-EPMC32182 | biostudies-literature | 2001
REPOSITORIES: biostudies-literature
Publications
Functional interaction between TRP4 and CFTR in mouse aorta endothelial cells.
Wei L L Freichel M M Jaspers M M Cuppens H H Cassiman J J JJ Droogmans G G Flockerzi V V Nilius B B
BMC physiology 20010515
<h4>Background</h4>This study describes the functional interaction between the putative Ca2+ channel TRP4 and the cystic fibrosis transmembrane conductance regulator, CFTR, in mouse aorta endothelium (MAEC).<h4>Results</h4>MAEC cells express CFTR transcripts as shown by RT-PCR analysis. Application of a phosphorylating cocktail activated a Cl- current with characteristics similar to those of CFTR mediated currents in other cells types (slow activation by cAMP, absence of rectification, block by ...[more]
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