Proteome driven re-evaluation and functional annotation of the Streptococcus pyogenes SF370 genome.

ABSTRACT:

Background

The genome data of Streptococcus pyogenes SF370 has been widely used by many researchers and provides a vast array of interesting findings. Nevertheless, approximately 40% of genes remain classified as hypothetical proteins, and several coding sequences (CDSs) have been unrecognized. In this study, we attempted a shotgun proteomic analysis with a six-frame database that was independent of genome annotation.

Results

Nine proteins encoded by novel ORFs were found by shotgun proteomic analysis, and their specific mRNAs were verified by reverse transcriptional PCR (RT-PCR). We also provided functional annotations for hypothetical genes using proteomic analysis from three different culture conditions that were separated into three fractions: supernatant, soluble, and i

SUBMITTER: Okamoto A 

PROVIDER: S-EPMC3224786 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature