Stromal interaction essential for vascular endothelial growth factor A-induced tumour growth via transforming growth factor-β signalling.

ABSTRACT:

Background

High vascular endothelial growth factor (VEGFA) levels at the time of diagnosis confer a worse prognosis to multiple malignancies. Our aim was to investigate the role of VEGFA in promoting tumour growth through interaction with its environment.

Methods

HL-60 cells were transduced with VEGFA165 or control vector using retroviral constructs. Control cells (n=7) or VEGFA165 cells (n=7) were subcutaneously injected into NOD/SCID mice. Immunohistochemistry of markers for angiogenesis (CD31) and cell proliferation (Ki67) and gene expression profiling of tumours were performed. Paracrine effects were investigated by mouse-specific cytokine arrays.

Results

In vivo we observed a twofold increase in tumour weight when VEGFA165 was overexpressed (P=0.001), combined wi

SUBMITTER: Weidenaar AC 

PROVIDER: S-EPMC3251883 | biostudies-literature | 2011 Dec

REPOSITORIES: biostudies-literature