Lag phase is a distinct growth phase that prepares bacteria for exponential growth and involves transient metal accumulation.

ABSTRACT: Lag phase represents the earliest and most poorly understood stage of the bacterial growth cycle. We developed a reproducible experimental system and conducted functional genomic and physiological analyses of a 2-h lag phase in Salmonella enterica serovar Typhimurium. Adaptation began within 4 min of inoculation into fresh LB medium with the transient expression of genes involved in phosphate uptake. The main lag-phase transcriptional program initiated at 20 min with the upregulation of 945 genes encoding processes such as transcription, translation, iron-sulfur protein assembly, nucleotide metabolism, LPS biosynthesis, and aerobic respiration. ChIP-chip revealed that RNA polymerase was not "poised" upstream of the bacterial genes that are rapidly induced at the beginning of lag phase, sug

SUBMITTER: Rolfe MD 

PROVIDER: S-EPMC3264077 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature