Ribosomal route to small-molecule diversity.

ABSTRACT: The cyanobactin ribosomal peptide (RP) natural product pathway was manipulated to incorporate multiple tandem mutations and non-proteinogenic amino acids, using eight heterologous components simultaneously expressed in Escherichia coli . These studies reveal the potential of RPs for the rational synthesis of complex, new small molecules over multiple-step biosynthetic pathways using simple genetic engineering.

SUBMITTER: Tianero MD 

PROVIDER: S-EPMC3268757 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature