Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay.
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ABSTRACT:
SUBMITTER: Dunams D
PROVIDER: S-EPMC3294457 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Publications
Simultaneous detection of infectious human echoviruses and adenoviruses by an in situ nuclease-resistant molecular beacon-based assay.
Dunams Daniela D Sarkar Payal P Chen Wilfred W Yates Marylynn V MV
Applied and environmental microbiology 20111222 5
A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection. ...[more]
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