Effect of intracellular lipid accumulation in a new model of non-alcoholic fatty liver disease.

ABSTRACT:

Background

In vitro exposure of liver cells to high concentrations of free fatty acids (FFA) results in fat overload which promotes inflammatory and fibrogenic response similar to those observed in patients with Non-Alcoholic Fatty Liver Disease (NAFLD) and Non-Alcoholic Steatohepatitis (NASH). Since the mechanisms of this event have not been fully characterized, we aimed to analyze the fibrogenic stimuli in a new in vitro model of NASH.

Methods

HuH7 cells were cultured for 24 h in an enriched medium containing bovine serum albumin and increasing concentrations of palmitic and oleic acid at a molar ratio of 1:2 (palmitic and oleic acid, respectively). Cytotoxic effect, apoptosis, oxidative stress, and production of inflammatory and fibrogenic cytokines were measured.

Resu

SUBMITTER: Chavez-Tapia NC 

PROVIDER: S-EPMC3313845 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature