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SiRNA off-target effects in genome-wide screens identify signaling pathway members.

ABSTRACT: We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection.

SUBMITTER: Buehler E 

PROVIDER: S-EPMC3361704 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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siRNA off-target effects in genome-wide screens identify signaling pathway members.

Buehler Eugen E   Khan Aly A AA   Marine Shane S   Rajaram Misha M   Bahl Amit A   Burchard Julja J   Ferrer Marc M  

Scientific reports 20120529

We introduce a method for analyzing small interfering RNA (siRNA) genetic screens based entirely on off-target effects. Using a screen for members of the Wnt pathway, we demonstrate that this method identifies known pathway components, some of which are not present in the screening library. This technique can be applied to siRNA screen results retroactively to confirm positives and identify genes missed using conventional methods for on-target gene selection. ...[more]

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