Imaging the material properties of bone specimens using reflection-based infrared microspectroscopy.

ABSTRACT: Fourier transform infrared microspectroscopy (FTIRM) is a widely used method for mapping the material properties of bone and other mineralized tissues, including mineralization, crystallinity, carbonate substitution, and collagen cross-linking. This technique is traditionally performed in a transmission-based geometry, which requires the preparation of plastic-embedded thin sections, limiting its functionality. Here, we theoretically and empirically demonstrate the development of reflection-based FTIRM as an alternative to the widely adopted transmission-based FTIRM, which reduces specimen preparation time and broadens the range of specimens that can be imaged. In this study, mature mouse femurs were plastic-embedded and longitudinal sections were cut at a thickness of 4 μm for transmissio

SUBMITTER: Acerbo AS 

PROVIDER: S-EPMC3364542 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature