Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34.

ABSTRACT: Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α(2)β(2)γ(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.

SUBMITTER: Rosier C 

PROVIDER: S-EPMC3370517 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature