ChAP-MS: a method for identification of proteins and histone posttranslational modifications at a single genomic locus.

ABSTRACT: The field of epigenomics has been transformed by chromatin immunoprecipitation approaches that provide for the localization of a defined protein or posttranslationally modified protein to specific chromosomal sites. While these approaches have helped us conceptualize epigenetic mechanisms, the field has been limited by the inability to define features such as the proteome and histone modifications at a specific genomic locus in an unbiased manner. We developed an unbiased approach whereby a unique native genomic locus was isolated, which was followed by high-resolution proteomic identification of specifically associated proteins and histone posttranslational modifications. This chromatin affinity purification with mass spectrometry (ChAP-MS) technique was used to specifically enrich a ~1,0

SUBMITTER: Byrum SD 

PROVIDER: S-EPMC3408609 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature